Anti-GABA antibody [5A9] (ab86186)
Key features and details
- Mouse monoclonal [5A9] to GABA
- Suitable for: IHC-P, ICC/IF, IHC-FoFr
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-GABA antibody [5A9]
See all GABA primary antibodies -
Description
Mouse monoclonal [5A9] to GABA -
Host species
Mouse -
Specificity
ab86186 is highly specific to GABA and does not react with other amino acid BSA conjugates with the exception of weak reaction against beta alanine BSA conjugate at the highest concentrations used. -
Tested applications
Suitable for: IHC-P, ICC/IF, IHC-FoFrmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
GABA coupled to BSA with glutaraldehyde
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General notes
ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
ab86186 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. -
Clonality
Monoclonal -
Clone number
5A9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-FoFr | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. |
Target
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Relevance
Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell. -
Cellular localization
Cell Membrane, Cytoplasmic, Plasma membrane and Secreted -
Alternative names
- 4 aminobutanoic acid antibody
- GABA antibody
- Gamma amino butyric acid antibody
- Gamma aminobutyric acid antibody
Images
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ab86186 staining GABA in the Brains of 2-5 days old moths (Manduca) by ICC/IF (Immunocytochemistry/immunofluorescence). The head capsules were fixed with 4% paraformaldehyde, washed in PBS and embedded in Low melting point Agarose and section to 75 μm thickness.Section were blocked with 2% BSA(IgG-free) . Samples were incubated with primary antibody (1/500 in PBS).
Double labeling against the Ms5HT1A receptor (green) and GABA (magenta) revealed a subset of Ms5HT1A-ir cell bodies co-localizing GABA (arrows). Scale bar = 100 μm.
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Immunohistochemical analysis of human brain tissue labeling GABA with ab86186 at 1:20 dilution. Sample was incubated with primary antibody for 60 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab86186 has been referenced in 11 publications.
- Scheuer T et al. Neonatal Oxidative Stress Impairs Cortical Synapse Formation and GABA Homeostasis in Parvalbumin-Expressing Interneurons. Oxid Med Cell Longev 2022:8469756 (2022). PubMed: 35663195
- Jia X et al. Activation of spinal PDGFRβ in microglia promotes neuronal autophagy via p38 MAPK pathway in morphine-tolerant rats. J Neurochem 158:373-390 (2021). PubMed: 33950542
- Yu XJ et al. Effects of Nrf1 in Hypothalamic Paraventricular Nucleus on Regulating the Blood Pressure During Hypertension. Front Neurosci 15:805070 (2021). PubMed: 34938159
- Trujillo CA et al. Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids. EMBO Mol Med 13:e12523 (2021). PubMed: 33501759
- Chéreau R et al. Dynamic perceptual feature selectivity in primary somatosensory cortex upon reversal learning. Nat Commun 11:3245 (2020). PubMed: 32591523