Overview

  • Product nameAnti-GABA antibody
    See all GABA primary antibodies
  • Description
    Guinea pig polyclonal to GABA
  • SpecificityRecognizes GABA. Staining was blocked by preabsorbing with 100uM GABA conjugated to glutaraldehyde. 500uM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining.
  • Tested applicationsSuitable for: Electron Microscopy, ICC/IF, IHC-FoFr, IHC-Frmore details
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH via glutaraldehyde.

  • General notes

    Without colchicine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • Primary antibody notesWithout colchine pretreatment well stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy Use at an assay dependent dilution. PubMed: 20206640
ICC/IF 1/1000.
IHC-FoFr 1/1000.
IHC-Fr 1/1500.

Target

  • RelevanceGamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
  • Cellular localizationCell Membrane, Cytoplasmic, Plasma membrane and Secreted
  • Alternative names
    • 4 aminobutanoic acid antibody
    • GABA antibody
    • Gamma amino butyric acid antibody
    • Gamma aminobutyric acid antibody

References for Anti-GABA antibody (ab17413)

This product has been referenced in:
  • Petrini EM  et al. Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP. Nat Commun 5:3921 (2014). Read more (PubMed: 24894704) »
  • Stephan AH  et al. A dramatic increase of C1q protein in the CNS during normal aging. J Neurosci 33:13460-74 (2013). Read more (PubMed: 23946404) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Antigen retrieval step None
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 09 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (LDT brain slices)
Specification LDT brain slices
Permeabilization Yes - TritonX
Fixative PFA/Gluteraldehyde
Username

Jason Teem

Verified customer

Submitted Sep 10 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample Chicken Tissue sections (chicken hindbrain)
Specification chicken hindbrain
Permeabilization Yes - 0.1% PBS-TritonX
Fixative Paraformaldehyde
Username

Dr. Karli Montague

Verified customer

Submitted Mar 26 2014


I can confirm that ab17413 anti-GABA antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentra...

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Thank you for bringing this to our attention. We have not tested the antibody on free-floating sections but your protocol is correct, assuming 10 minutes for permeabilization of these 50um sections was sufficient. We have one other complaint about this...

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Thank you for contacting us.

This antibody has been tested on GABAergic interneurons of mammalian samples, but we do not have testing or reports using hESC.

I hope this information is helpful to you. Please do not hesitate to contact ...

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Thank you for providing some further useful information.

Both antibodies would be suitable for the immunostaining. However, it seems that ab17413 is more popular amongst our customers since it has been characterized in a number of application...

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Thank you for contacting Abcam regarding ab17413. Regarding the tissue fixation, I have confirmed with the laboratory that yes, the combination of 4% paraformaldehyde / glutaraldehyde fixation is typically used in successful IHC staining.  Please f...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case f...

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Thank you for your enquiry. I am pleased to hear this antibody has worked well for you. The is some immunogen information for this antibody is on the datasheet: Chemical / Small Molecule conjugated to KLH via glutaraldehyde. GAB...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"