Overview

  • Product name
  • Description
    Rabbit polyclonal to GABA
  • Specificity
    Using a conjugate GABA-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
    CompoundCross-reactivity ratio (a)
    GABA-G-BSA1
    beta-Alanine-G-BSA1/10000
    Glycine-G-BSA1/20000
    D-Aspartate-G-BSA1/100000
    D-Glutamate-G-PPL1/>10000
    (a): GABA-G-BSA concentration/unconjugated or conjugated aminoacid concentration at half displacement. G = Glutaraldehyde, BSA = bovine serum albumin.
  • Tested applications
    Suitable for: ELISA, IHC-Frmore details
  • Immunogen

    Chemical/ Small Molecule by a Glutaraldehyde linker.

  • General notes


    The antiserum was tested using the free floating PAP technique on rat hippocampus and cerebellum. The anti-conjugated GABA antibodies gave a good staining between 1/2000 - 1/5000 dilution in these areas.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Primary antibody notes
    The antiserum was tested using the free floating PAP technique on rat hippocampus and cerebellum. The anti-conjugated GABA antibodies gave a good staining between 1/2000 - 1/5000 dilution in these areas.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
  • Cellular localization
    Cell Membrane, Cytoplasmic, Plasma membrane and Secreted
  • Alternative names
    • 4 aminobutanoic acid antibody
    • GABA antibody
    • Gamma amino butyric acid antibody
    • Gamma aminobutyric acid antibody

References

ab6443 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Ab6443 doesn't recognize GABA free.

Yes, see step 1 in immunohisto and cytochemistry protocol (link to protocol is on top right corner of datasheet).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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