Overview

  • Product nameAnti-GABA antibody
    See all GABA primary antibodies
  • Description
    Rabbit polyclonal to GABA
  • Specificityab8891 targets conjugated GABA (Gamma-Aminobutyric acid), it does not recognize free GABA.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IHC-FoFrmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to GABA. Synthetic GABA conjugated to bovine serum albumin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, ddH20
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Fixation of tissue for use with these antibodies should be done with glutaraldehyde. The use of paraformaldehyde in conjunction with glutaraldehyde may improve staining results.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 17394161

Target

  • RelevanceGamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell.
  • Cellular localizationCell Membrane, Cytoplasmic, Plasma membrane and Secreted
  • Alternative names
    • 4 aminobutanoic acid antibody
    • GABA antibody
    • Gamma amino butyric acid antibody
    • Gamma aminobutyric acid antibody

Anti-GABA antibody images

  • Immunohistochemical analysis of rat brain tissue, staining GABA with ab8891.

    Slices were fixed in a 2% glutaraldehyde, 2% formaldehyde solution before being blocked for 2 h at room temperature in PBS with 0.3% Tween-20, 0.2% BSA and 5% normal goat serum. Samples were incubated with primary antibody (1/200) before detection with a fluorescence-conjugated goat anti-rabbit secondary antibody.
  • Immunofluorescent analysis of Human embryonic stem cells, staining GABA with ab8891.

    Cells were fixed with paraformaldehyde before blocking with 10% goat serum, 1% BSA, 0.1% Triton X-100 for 1 hour. Samples were stained with primary antibody (1/500) overnight at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

References for Anti-GABA antibody (ab8891)

This product has been referenced in:

See all 6 Publications for this product

Product Wall

The optimal incubation time will depend on how your tissue sections are prepared.

The protocol used for the stain of rat brain tissue shown on the datasheet is from this reference:

Margolis EB et al. Identification of rat ventral tegm...

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step None
Sample Mouse Tissue sections (early LDT coronal sections)
Specification early LDT coronal sections
Permeabilization Yes - TritonX-100
Fixative Paraformaldehyde
Username

Jason Teem

Verified customer

Submitted Jan 08 2014

Thank you for contacting us.
The antibody has been tested in western blot in conjunction with the antigen as a positive control, in which case the band would is ˜67 kDa as the antigen is made with BSA. Free GABA will not be an option. Whatever is l...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case f...

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Thank you for your enquiry. According to the reference available for ab8891, they injected rabbits with a GABA-glutaraldehyde-lysine conjugate. I am still awaiting further information regarding ab17390. I hope this information helps. Please do ...

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The originator of this antibody has informed me that they do not know of any species ab8891 does NOT work in successfully. So, it should work in rat. Please let me know if your customer has any more questions.

This antibody has been tested and characterised for ELISA application. All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). I would suggest that perhaps yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"