The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/500. Predicted molecular weight: 106 kDa.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/50 - 1/100.
Application notesIs unsuitable for IP.
FunctionReceptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
Tissue specificityHighly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
DomainAlpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
ab75838, at a 1/100 dilution, staining GABA B Receptor 2 in paraffin embedded human brain tissue by Immunohistochemistry.
Western blot - GABA B Receptor 2 antibody [EP2411Y] (ab75838)
Anti-GABA B Receptor 2 antibody [EP2411Y] (ab75838) at 1/500 dilution + human cerebellum lysate at 10 µg
Secondary goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 106 kDa Observed band size : 106 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GABA B Receptor 2 antibody [EP2411Y] (ab75838)Image from Cuny H et al., J Biol Chem. 2012 Jul 6;287(28):23948-57. Epub 2012 May 21.; Fig 2.; doi: 10.1074/jbc.M112.342998; July 6, 2012, The Journal of Biological Chemistry, 287, 23948-23957.
Immunofluorescence analysis of GABA B Receptor 2 using ab75838 at 1/400 dilution. Left panel = normal rat DRG neurons. Right panel = rat DRG neurons transfected with siRNAs to disrupt GABA B Receptor 2.
Flow Cytometry-Anti-GABA B Receptor 2 antibody [EP2411Y](ab75838)
Overlay histogram showing SH-SY5Y cells stained with ab75838 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75838, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-GABA B Receptor 2 antibody [EP2411Y] (ab75838)
This product has been referenced in:
Banerjee J et al. Differential modulation of nicotine-induced gemcitabine resistance by GABA receptor agonists in pancreatic cancer cell xenografts and in vitro. BMC Cancer14:725 (2014).
Read more (PubMed: 25260978) »
Cuny H et al. ?-Aminobutyric Acid Type B (GABAB) Receptor Expression Is Needed for Inhibition of N-type (Cav2.2) Calcium Channels by Analgesic a-Conotoxins. J Biol Chem287:23948-57 (2012).
Read more (PubMed: 22613715) »