The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/500 - 1/1000. Detects a band of approximately 105 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionReceptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
Tissue specificityHighly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.
DomainAlpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 1.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane. Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane.
Immunohistochemical analysis of phosphorylated GABA B Receptor 2 expression on paraffin-embedded Human brain tissue using 1/100 ab68426. Heat mediated antigen retrieval was performed, and an HRP-conjugated polymerized secondary antibody was used.
References for Anti-GABA B Receptor 2 (phospho S893) antibody [EP2318Y] (ab68426)
has not yet been referenced specifically in any publications.
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