Anti-GABA Transporter 1 / GAT 1 antibody (ab72448)

Overview

  • Product nameAnti-GABA Transporter 1 / GAT 1 antibody
    See all GABA Transporter 1 / GAT 1 primary antibodies
  • Description
    Rabbit polyclonal to GABA Transporter 1 / GAT 1
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Peptide corresponding to amino acid residues from the C-terminal region of rat GABA Transporter 1/ GAT 1

  • Positive control
    • rat hippocampal homogenate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

Target

  • FunctionTerminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.
  • Sequence similaritiesBelongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.
  • DomainThe PDZ domain-binding motif is involved in the interaction with MPP5.
  • Cellular localizationCell membrane. Membrane. Localized at the plasma membrane and in a subset of intracellular vesicles. Localized at the presynaptic terminals of interneurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • GABATHG antibody
    • GABATR antibody
    • GABT 1 antibody
    • GABT1 antibody
    • GAT-1 antibody
    • GAT1 antibody
    • SC6A1_HUMAN antibody
    • Slc6a1 antibody
    • Sodium and chloride dependent GABA transporter 1 antibody
    • Sodium- and chloride-dependent GABA transporter 1 antibody
    • Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody
    • Solute carrier family 6 member 1 antibody
    see all

Anti-GABA Transporter 1 / GAT 1 antibody images

  • Anti-GABA Transporter 1 / GAT 1 antibody (ab72448) at 1/1000 dilution + rat hippocampal homogenate

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • ab72448 staining GABA Transporter 1 / GAT 1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 5% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/500 in 5% serum) for 16 hours at 4°C. A DyLight®-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-GABA Transporter 1 / GAT 1 antibody (ab72448)

ab72448 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Your credit note/refund ID is I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchas...

Read More

Thank you for your message and for providing this further information.

From our discussion and from reviewing your protocol and images it appears likely that in this case the issue lies in the vial of this product itself. I appreciate the ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Hippocampus)
Permeabilization Yes - 0.1% PBS-Triton X
Specification Hippocampus
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain (hippocampus))
Permeabilization No
Specification Brain (hippocampus)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Mr. Yahyah Aman

Verified customer

Submitted Jan 22 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 04 2013

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on th...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"