Anti-GABA Transporter 3 / GAT 3 antibody (ab431)

Overview

  • Product nameAnti-GABA Transporter 3 / GAT 3 antibody
    See all GABA Transporter 3 / GAT 3 primary antibodies
  • Description
    Rabbit polyclonal to GABA Transporter 3 / GAT 3
  • SpecificityThis antibody reacts with a 74 kDa protein from CNS samples.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Chicken
    Predicted to work with: Mouse, Human
  • Immunogen

    15 residue C-terminal synthetic peptide (Rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
IHC-Fr 1/50 - 1/500.
WB 1/200 - 1/5000.

Target

References for Anti-GABA Transporter 3 / GAT 3 antibody (ab431)

This product has been referenced in:
  • Aligny C  et al. Ketamine alters cortical integration of GABAergic interneurons and induces long-term sex-dependent impairments in transgenic Gad67-GFP mice. Cell Death Dis 5:e1311 (2014). IHC (PFA fixed), WB ; Mouse . Read more (PubMed: 24991763) »
  • Gosselin RD  et al. Extracellular microvesicles from astrocytes contain functional glutamate transporters: regulation by protein kinase C and cell activation. Front Cell Neurosci 7:251 (2013). WB ; Rat . Read more (PubMed: 24368897) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Guinea pig Tissue sections (Brain_Region B)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrtae buffer ph 6
Permeabilization No
Specification Brain_Region B
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative formalin
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Verified customer

Submitted Apr 07 2016

Vielen Dank für Ihre Anfrage.

Um es gleich zu sagen: Die Chance, dass unsere Antikörper in EM getestet wurden, ist leider relativ gering. Das heißt, wir können die Anwendung nicht garantieren. Ich kann Ihnen aber einen Test- Rabatt ausstelle...

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The GAT3 antibody ab431 detected a band at 74 kDa in CNS samples, as noted on the datasheet. The precursor of the protein, before any post-translation modifications has a predicted molecular weight of about 70 kD...

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These antibodies will work on both applications : frozen sections and paraffin embedded sections.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"