Anti-GABARAP antibody (ab1398)
Key features and details
- Rabbit polyclonal to GABARAP
- Suitable for: ELISA, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GABARAP antibody
See all GABARAP primary antibodies -
Description
Rabbit polyclonal to GABARAP -
Host species
Rabbit -
Specificity
This antibody recognizes human GABARAP (14 kD). -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide:
RSEGEKIRKKYPDRVPV
, corresponding to amino acids 15-31 of Human GABARAP. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
|
Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. -
Tissue specificity
Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the ATG8 family. -
Post-translational
modificationsThe precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II. -
Cellular localization
Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 11337 Human
- Entrez Gene: 56486 Mouse
- Omim: 605125 Human
- SwissProt: O95166 Human
- SwissProt: Q9DCD6 Mouse
- Unigene: 647421 Human
- Unigene: 272460 Mouse
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Alternative names
- ATG8A antibody
- FLC 3B antibody
- FLC3B antibody
see all
Datasheets and documents
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Datasheet download
References (4)
ab1398 has been referenced in 4 publications.
- Sujkowski A et al. Endurance exercise ameliorates phenotypes in Drosophila models of spinocerebellar ataxias. Elife 11:N/A (2022). PubMed: 35170431
- Li L et al. Proteomic analysis of differentially expressed proteins in kidneys of brain dead rabbits. Mol Med Rep 16:215-223 (2017). WB ; Rabbit . PubMed: 28534953
- Ning HJ et al. Propofol reduces hypoxia-induced autophagic cell death through downregulating HIF 1a in alveolar epithelial type II cells of rats. Mol Med Rep 16:1509-1515 (2017). PubMed: 28586054
- Belichenko PV et al. Excitatory-inhibitory relationship in the fascia dentata in the Ts65Dn mouse model of Down syndrome. J Comp Neurol 512:453-66 (2009). Mouse . PubMed: 19034952