Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

Overview

  • Product nameAnti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]
    See all GABARAP+GABARAPL1+GABARAPL2 primary antibodies
  • Description
    Rabbit monoclonal [EPR4805] to GABARAP+GABARAPL1+GABARAPL2
  • Specificityab109364 will also recognize related targets GABARAPL1 and GABARAPL2: GABARAPL1: pBLAST 100% immunogen homology AND Abreview 38385 WB data GABARAPL2: pBLAST 93% homology (only 1aa gap of 14aa) AND Abreview 38385 WB data
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Silk worm
  • Immunogen

    corresponding to Human GABARAP+GABARAPL1+GABARAPL2 aa 1-100.

  • Positive control
    • Fetal kidney lysate, fetal brain lysate, SH-SY5Y cell lysate, HepG2 cell lysate, human brain tissue, HeLa cells.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P 1/500 - 1/1000. Antigen retriecal is recommended
ICC/IF 1/100 - 1/500.
  • Application notesIs unsuitable for IP.
  • Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] images

    • ab109364 staining GABARAP+GABARAPL1+GABARAPL2 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling GABARAP+GABARAPL1+GABARAPL2 with purified ab109364 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    • All lanes : Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364) at 1/1000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 14 kDa
    • ab109364, at 1/500, staining GABARAP+GABARAPL1+GABARAPL2 in Human brain tissue by immunohistochemistry.

    • ab109364, at 1/100, staining HeLa cells by immunofluorescence.

    References for Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab109364)

    This product has been referenced in:
    • Cappelletti C  et al. Autophagy, inflammation and innate immunity in inflammatory myopathies. PLoS One 9:e111490 (2014). Radioimmunoprecipitation ; Human . Read more (PubMed: 25365350) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HepG2 cells)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification HepG2 cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 13 2017

    Application Western blot
    Sample Human Tissue lysate - whole (skeletal muscle tissue)
    Gel Running Conditions Reduced Denaturing (4-12%)
    Loading amount 20 µg
    Specification skeletal muscle tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 12 2015

    Application Western blot
    Loading amount 40 µg
    Gel Running Conditions Reduced Denaturing (12%)
    Sample Silk worm (Bombyx mori) Tissue lysate - whole (Midgut)
    Specification Midgut
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 02 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (13%)
    Sample Human Cell lysate - whole cell (SH-SY5Y cells stably expressing GFP, GFP-GABARAP,)
    Specification SH-SY5Y cells stably expressing GFP, GFP-GABARAP,
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Dr. George Allen

    Verified customer

    Submitted Sep 26 2013

    Application IHC - Wholemount
    Sample Fruit fly (Drosophila melanogaster) Tissue (fat body)
    Specification fat body
    Username

    Abcam user community

    Verified customer

    Submitted Sep 19 2013

    Thank you very much for your interest our AbTrial program.



    I performed a comparison of the immunogens for our GABARAP antibodies, and the identity between the immunogen of ab109364 and the silkworm protein looks very promising with ...

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    Thank you for your reply.

    I wish you good luck with the live reporter experiments.

    I will be happy to issue the discount code for you now, but I think it would be better to do this when you know that you will use it. The discount co...

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    Thank you very much for your interest in our antibodies.

    According to a quick blast check the immunogen of ab1398 has a homology with Drosophila melanogaster of 82%. We generally expect cross reaction of an antibody from 85% homology and mor...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"