Overview

  • Product nameAnti-GABARAPL1 antibody
    See all GABARAPL1 primary antibodies
  • Description
    Rabbit polyclonal to GABARAPL1
  • Specificityab77952 recognizes GABARAPL1. By Western blot the antibody recognizes a band at ~14 kDa in mouse brain lysate. The band is completely abolished after incubating the antibody with the immunogen peptide. Due to sequence homology of the targeted region, this antibody will very likely also recongize the GABARAP protein.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse GABARAPL1 aa 18-32.
    Sequence:

    GEKIRKKYPDRVPVI

  • Positive control
    • Mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).

Target

  • FunctionUbiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
  • Tissue specificityUbiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
  • Sequence similaritiesBelongs to the ATG8 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.
    The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Endoplasmic reticulum. Golgi apparatus. Cytoplasmic vesicle > autophagosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • APG8 like antibody
    • APG8L antibody
    • ATG8 antibody
    • ATG8B antibody
    • ATG8L antibody
    • Early estrogen regulated protein antibody
    • Early estrogen-regulated protein antibody
    • GABA antibody
    • GABA(A) receptor associated protein like 1 antibody
    • GABA(A) receptor-associated protein-like 1 antibody
    • GABARAPL1 a antibody
    • GABARAPL1 antibody
    • Gamma aminobutyric acid receptor associated protein like 1 antibody
    • Gamma-aminobutyric acid receptor-associated protein-like 1 antibody
    • GBRL1_HUMAN antibody
    • GEC 1 antibody
    • GEC-1 antibody
    • GEC1 antibody
    • Glandular epithelial cell protein 1 antibody
    see all

Anti-GABARAPL1 antibody images

  • Anti-GABARAPL1 antibody (ab77952) at 1 µg/ml + mouse brain lysate

    Predicted band size : 14 kDa
    Observed band size : 14 kDa

References for Anti-GABARAPL1 antibody (ab77952)

ab77952 has not yet been referenced specifically in any publications.

Product Wall

The immunogen of ab77952 corresponds to amino acids 18-32 of the human GABARAPL1 which is the same as in GABARAP. This antibody will therefore recongize GABARAPL1 as well as GABARAP.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"