Anti-GABARAPL1 antibody (ab86503)

Overview

  • Product nameAnti-GABARAPL1 antibody
    See all GABARAPL1 primary antibodies
  • Description
    Rabbit polyclonal to GABARAPL1
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human GABARAPL1 aa 36-85 (internal sequence).
    Sequence:

    APKARVPDLDKRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIP

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionUbiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
  • Tissue specificityUbiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
  • Sequence similaritiesBelongs to the ATG8 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.
    The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Endoplasmic reticulum. Golgi apparatus. Cytoplasmic vesicle > autophagosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • APG8 like antibody
    • APG8L antibody
    • ATG8 antibody
    • ATG8B antibody
    • ATG8L antibody
    • Early estrogen regulated protein antibody
    • Early estrogen-regulated protein antibody
    • GABA antibody
    • GABA(A) receptor associated protein like 1 antibody
    • GABA(A) receptor-associated protein-like 1 antibody
    • GABARAPL1 a antibody
    • GABARAPL1 antibody
    • Gamma aminobutyric acid receptor associated protein like 1 antibody
    • Gamma-aminobutyric acid receptor-associated protein-like 1 antibody
    • GBRL1_HUMAN antibody
    • GEC 1 antibody
    • GEC-1 antibody
    • GEC1 antibody
    • Glandular epithelial cell protein 1 antibody
    see all

Anti-GABARAPL1 antibody images

  • Anti-GABARAPL1 antibody (ab86503) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 14 kDa

References for Anti-GABARAPL1 antibody (ab86503)

ab86503 has not yet been referenced specifically in any publications.

Product Wall

I looked at the alignment of the immunogen sequences of our antibodies against GABARAPL1 with GABARAP. The exact immunogen sequences of ab86497 and ab86503 are unfortunately not available, but the laboratory did an alignment: Blast homology shows that...

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