Anti-GABARAPL1 antibody (ab94996)

Overview

  • Product nameAnti-GABARAPL1 antibody
    See all GABARAPL1 primary antibodies
  • Description
    Rabbit polyclonal to GABARAPL1
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human GABARAPL1 recombinant protein.

  • Positive control
    • HeLa cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab94996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.
IHC-P 1/50 - 1/100.
ELISA Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 14 kDa.

Target

  • FunctionUbiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
  • Tissue specificityUbiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
  • Sequence similaritiesBelongs to the ATG8 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.
    The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Endoplasmic reticulum. Golgi apparatus. Cytoplasmic vesicle > autophagosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • APG8 like antibody
    • APG8L antibody
    • ATG8 antibody
    • ATG8B antibody
    • ATG8L antibody
    • Early estrogen regulated protein antibody
    • Early estrogen-regulated protein antibody
    • GABA antibody
    • GABA(A) receptor associated protein like 1 antibody
    • GABA(A) receptor-associated protein-like 1 antibody
    • GABARAPL1 a antibody
    • GABARAPL1 antibody
    • Gamma aminobutyric acid receptor associated protein like 1 antibody
    • Gamma-aminobutyric acid receptor-associated protein-like 1 antibody
    • GBRL1_HUMAN antibody
    • GEC 1 antibody
    • GEC-1 antibody
    • GEC1 antibody
    • Glandular epithelial cell protein 1 antibody
    see all

Anti-GABARAPL1 antibody images

  • Anti-GABARAPL1 antibody (ab94996) at 1/100 dilution + Hela cell line lysate at 35 µg

    Predicted band size : 14 kDa
  • All lanes : Anti-GABARAPL1 antibody (ab94996) at 1/500 dilution

    Lane 1 : Human SH-SY5Y whole cell lysate - expressing GFP
    Lane 2 : Human SH-SY5Y whole cell lysate - expressing GFP-GABARAP
    Lane 3 : Human SH-SY5Y whole cell lysate - expressing GFP-GABARAPL1
    Lane 4 : Human SH-SY5Y whole cell lysate - epressing GFP-GABARAPL2

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible non-specific binding).

    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by George Allen

    See Abreview

  • Immunohistochemistry analysis of formalin-fixed,paraffin-embedded Human brain tissue labeling GABARAPL1 with ab94996 at 1/50 dilution which was peroxidase-conjugated to the secondary antibody,followed by DAB staining.

  • Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References for Anti-GABARAPL1 antibody (ab94996)

ab94996 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (SH-SY5Y cells)
Specification SH-SY5Y cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. George Allen

Verified customer

Submitted Sep 26 2013

Ab94996 has been produced with as immungen the whole molecule. The GABARAPL1 protein has 86% identity with the GABARAP protein. It is therefore difficult to make a prediction. Unfortunately, this antibody has not been tested by the laboratory for its c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"