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Synthetic peptide, corresponding to residues in Human GABARAPL2.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab126607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: GABARAPL2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human tonsil lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab126607 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab126607 was shown to specifically react with GABARAPL2 when GABARAPL2 knockout samples were used. Wild-type and GABARAPL2 knockout samples were subjected to SDS-PAGE. ab126607 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"