Overview

  • Product name
  • Description
    Rabbit polyclonal to GABPA
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Cow, Dog, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GABPA.

  • Positive control
    • This antibody gave a positive signal in NIH3T3 whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • E4TF 1A antibody
    • E4TF1 60 antibody
    • E4TF1A antibody
    • GA binding protein alpha chain antibody
    • GA binding protein transcription factor alpha subunit 60kDa antibody
    • GA binding protein transcription factor alpha subunit antibody
    • GA-binding protein alpha chain antibody
    • GABP A antibody
    • GABP alpha subunit antibody
    • GABP subunit alpha antibody
    • Gabpa antibody
    • GABPA_HUMAN antibody
    • Human nuclear respiratory factor 2 subunit alpha antibody
    • NFT 2 antibody
    • NFT2 antibody
    • NRF 2 antibody
    • NRF 2A antibody
    • NRF2 antibody
    • NRF2A antibody
    • Nuclear respiratory factor 2 alpha subunit antibody
    • Nuclear respiratory factor 2 subunit alpha antibody
    • RCH04A07 antibody
    • Transcription factor E4TF1 60 antibody
    • Transcription factor E4TF1-60 antibody
    see all

Images

  • Anti-GABPA antibody (ab109328) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

References

ab109328 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109328.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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