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Product name
Anti-GABPA antibody
See all GABPA products (6) ...
Description
Rabbit polyclonal to GABPA
Specificity
ab22838 reacts with GA Binding Protein a chain (GABPA).
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Cow, Dog, Pig, Chimpanzee, Fruit fly (Drosophila melanogaster), Zebrafish
Immunogen
A 14-22 amino acid region within synthetic peptide: QRKNKPTMNY EKLSRALRYY YDGDMICKVQ GKRFVYKFVC DLKTLIGYSA, corresponding to C terminal amino acids 361-410 of Human GABPA.
QRKNKPTMNYEKLSRALRYYYDGDMICKVQGKRFVYKFVC DLKTLIGYSA
Positive control
Jurkat cell lysate and human kidney tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 60 kDa.Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Function
Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ06546
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- GABP alpha subunit antibody
- Nuclear respiratory factor 2 subunit alpha antibody
- Transcription factor E4TF1 60 antibody
see all
Database links
- Entrez Gene: 281775 Cow
- Entrez Gene: 2551 Human
- Entrez Gene: 14390 Mouse
- Entrez Gene: 363735 Rat
- Entrez Gene: 58110 Zebrafish
- Omim: 600609 Human
- SwissProt: Q0VC98 Cow
- SwissProt: Q8IYS3 Human
see all
Anti-GABPA antibody images:
Western blot - GABPA antibody (ab22838)
Anti-GABPA antibody (ab22838) at 2 µg/ml + Jurkat cell lysate with 5% skim milk in PBS
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - GABPA antibody (ab22838)
Immunohistochemistry detection of GABPA in paraffin embedded human kidney tissue section. ab22838 used at ug/ml. Arrows indicate the positively labelled renal corpuscule cells.
References for Anti-GABPA antibody (ab22838)
This product has been referenced in:
- Leuenberger N et al. Sumoylated PPARalpha mediates sex-specific gene repression and protects the liver from estrogen-induced toxicity in mice. J Clin Invest : (2009). ChIP; Mouse.Read more (PubMed: 19729835) »
- Izumi T et al. Activation of synoviolin promoter in rheumatoid synovial cells by a novel transcription complex of interleukin enhancer binding factor 3 and GA binding protein alpha. Arthritis Rheum 60:63-72 (2009). EMSA; Human, Mouse.Read more (PubMed: 19116932) »
See all 2 publications for this product
Publishing research using ab22838? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"