Anti-GABPB2 antibody (ab88746)

Overview

  • Product name
    Anti-GABPB2 antibody
  • Description
    Mouse polyclonal to GABPB2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length Human protein (NP_005245.2)

  • Positive control

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1 x PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
IP
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is not essential but may optimise staining. Microwave method recommended.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene.
    • Sequence similarities
      Contains 5 ANK repeats.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BABPB2 antibody
      • E4TF1 antibody
      • E4TF1-47 antibody
      • E4TF1-53 antibody
      • E4TF1B antibody
      • GA binding protein transcription factor beta subunit 1 transcript variant gamma 2 antibody
      • GA binding protein transcription factor, beta subunit 1 antibody
      • GA binding protein transcription factor, beta subunit 2 antibody
      • GA-binding protein subunit beta-1 antibody
      • GABP subunit beta-1 antibody
      • GABP subunit beta-2 antibody
      • GABP1_HUMAN antibody
      • GABPB antibody
      • GABPB-1 antibody
      • GABPB-2 antibody
      • GABPB1 antibody
      • GABPB2 antibody
      • NRF2B1 antibody
      • NRF2B2 antibody
      • Nuclear respiratory factor 2 antibody
      • Transcription factor E4TF1-47 antibody
      • Transcription factor E4TF1-53 antibody
      see all

    Anti-GABPB2 antibody images

    • Anti-GABPB2 antibody (ab88746) at 1 µg/ml + PC-12 cell lysate at 50 µg

      Predicted band size : 42 kDa
    • Anti-GABPB2 antibody (ab88746) at 1 µg/ml + HeLa nuclear extracts at 50 µg

      Predicted band size : 42 kDa
    • Anti-GABPB2 antibody (ab88746) at 1 µg/ml + Jurkat cell lysate at 50 µg

      Predicted band size : 42 kDa
    • All lanes : Anti-GABPB2 antibody (ab88746) at 1 µg/ml

      Lane 1 : lysate from transfected 293T cells
      Lane 2 : lysate from non-transfected 293T cells

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 42 kDa
    • ab88746 at 3 µg/ml staining GABPB2 in formalin-fixed, paraffin-embedded Human endometrium by immunohistochemistry.
    • ab88746 at 10 µg/ml staining GABPB2 in PFA-fixed, permeabilised HeLa cells by immunofluorescence.

    References for Anti-GABPB2 antibody (ab88746)

    ab88746 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human cancer cell lines)
    Loading amount
    20 µg
    Specification
    Human cancer cell lines
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 22 2012

    Thank you for your enquiry and your interest in our products.

    I can confirm that ab97928 LOXL1 peptide was used to raise the antibody against LOXL1 (ab87748) and it can be used as blocking peptide with studies using http://www.abcam.com/index...

    Read More

    I apologize for being late in getting back to you.
    The peptide ab97928 is in stock. You can place an order any time.
    I hope this info will be helpful. Should you have any other question please do not hesitate to contact me.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (9L, PC12, RIE-1)
    Loading amount
    20 µg
    Specification
    9L, PC12, RIE-1
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 30 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HEK293 cells stably transfected with FLAG-GABPB2)
    Total protein in input
    20 µg
    Specification
    HEK293 cells stably transfected with FLAG-GABPB2
    Immuno-precipitation step
    Protein G
    Username

    Abcam user community

    Verified customer

    Submitted Jun 23 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF F9 GC-1 NIH-3T3)
    Loading amount
    20 µg
    Specification
    MEF F9 GC-1 NIH-3T3
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 16 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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