Overview

  • Product name
  • Description
    Rabbit polyclonal to GABRQ
  • Specificity
    This antibody recognizes GABRQ
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: KFHFEFSSAV PEVVLNLFNC KNCANEAVVQ KILDRVLSRY DVRLRPNFGG, corresponding to amino acids 31-80 of Human GABRQ

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab49188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay was 1/12500.

WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 72 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, theta subunit. GABRQ gene is mapped to chromosome Xq28 in a cluster including the genes encoding the alpha 3 and epsilon subunits of the same receptor. This gene location is also the candidate region of 2 different neurologic diseases: early-onset parkinsonism (Waisman syndrome) and X linked mental retardation (MRX3).
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • gamma aminobutyric acid (GABA) receptor, theta antibody
    • Gamma aminobutyric acid receptor theta subunit precursor antibody
    • gamma aminobutyric acid receptor, theta antibody
    • THETA antibody

Images

  • Lane 1 : Marker
    Lane 2 : Anti-GABRQ antibody (ab49188) at 0.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 72 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Fatemi SH  et al. mRNA and protein expression for novel GABAA receptors ? and ?2 are altered in schizophrenia and mood disorders; relevance to FMRP-mGluR5 signaling pathway. Transl Psychiatry 3:e271 (2013). WB ; Human . Read more (PubMed: 23778581) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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