Overview

  • Product name
  • Description
    Rabbit polyclonal to GABRR2
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 144-193 (SNKSMTFDGR LVKKIWVPDV FFVHSKRSFT HDTTTDNIML RVFPDGHVLY) of human GABRR2 (NP_002034).

  • Positive control
    • Hela cell lysate IF/ICC: SKNSH cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Relevance
    GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the gaba/benzodiazepine receptors and opening an integral chloride channel. GABRR2 is a member of the rho subunit family of GABA receptors and could play a role in retinal neurotransmission.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • GABA receptor Rho 2 subunit antibody
    • GABA(A) receptor antibody
    • GABA(A) receptor subunit rho-2 antibody
    • GABA(C) receptor antibody
    • GABRR 2 antibody
    • Gamma aminobutyric acid receptor rho 2 subunit [Precursor] antibody
    • Gamma-aminobutyric acid (GABA) A receptor, rho 2 antibody
    • Gamma-aminobutyric acid receptor subunit rho-2 antibody
    • Rho 2 GABA receptor antibody
    see all

Images

  • ICC/IF image of ab83223 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83223, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Anti-GABRR2 antibody (ab83223) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Gel Concentration: 12%

References

This product has been referenced in:
  • Rosas-Arellano A  et al. A simple solution for antibody signal enhancement in immunofluorescence and triple immunogold assays. Histochem Cell Biol 146:421-30 (2016). Read more (PubMed: 27188756) »
  • Fatemi SH  et al. mRNA and protein expression for novel GABAA receptors ? and ?2 are altered in schizophrenia and mood disorders; relevance to FMRP-mGluR5 signaling pathway. Transl Psychiatry 3:e271 (2013). WB ; Human . Read more (PubMed: 23778581) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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