Anti-GAD65 antibody (ab112007)
Key features and details
- Goat polyclonal to GAD65
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GAD65 antibody
See all GAD65 primary antibodies -
Description
Goat polyclonal to GAD65 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig, Chimpanzee -
Immunogen
Synthetic peptide:
C-TLEDNEERMSRLSK
, corresponding to internal sequence amino acids 515-528 of Human GAD65. -
Positive control
- Human Brain, cerebellum and colon tissues; Human brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 65 kDa.
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IHC-P | (1) |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 65 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the production of GABA. -
Sequence similarities
Belongs to the group II decarboxylase family. -
Post-translational
modificationsPhosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering. -
Cellular localization
Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. - Information by UniProt
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Database links
- Entrez Gene: 466026 Chimpanzee
- Entrez Gene: 487107 Dog
- Entrez Gene: 2572 Human
- Entrez Gene: 14417 Mouse
- Entrez Gene: 396929 Pig
- Entrez Gene: 24380 Rat
- Omim: 138275 Human
- SwissProt: Q4PRC2 Dog
see all -
Alternative names
- 65 kDa glutamic acid decarboxylase antibody
- DCE 2 antibody
- DCE2 antibody
see all
Images
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ab112007, at 2.5µg/ml, staining GAD65 in formalin-fixed, paraffin-embedded Human brain, cerebellum tissue by Immunohistochemistry.
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ab112007, at 2.5µg/ml, staining GAD65 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry.
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Anti-GAD65 antibody (ab112007) at 0.1 µg/ml + Human brain lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 65 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab112007 has not yet been referenced specifically in any publications.