Anti-GAD65 antibody (ab112007)

Overview

  • Product name
  • Description
    Goat polyclonal to GAD65
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide:

    C-TLEDNEERMSRLSK

    , corresponding to internal sequence amino acids 515-528 of Human GAD65.

  • Positive control
    • Human Brain, cerebellum and colon tissues; Human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab112007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 65 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Catalyzes the production of GABA.
  • Sequence similarities
    Belongs to the group II decarboxylase family.
  • Post-translational
    modifications
    Phosphorylated; which does not affect kinetic parameters or subcellular location.
    Palmitoylated; which is required for presynaptic clustering.
  • Cellular localization
    Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane. Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
  • Information by UniProt
  • Database links
  • Alternative names
    • 65 kDa glutamic acid decarboxylase antibody
    • DCE 2 antibody
    • DCE2 antibody
    • DCE2_HUMAN antibody
    • GAD 2 antibody
    • GAD 65 antibody
    • GAD-2 antibody
    • GAD-65 antibody
    • Gad2 antibody
    • Glutamate decarboxylase 2 (pancreas) antibody
    • Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody
    • Glutamate decarboxylase 2 antibody
    • Glutamate Decarboxylase 65 antibody
    • Glutamate decarboxylase 65 kDa isoform antibody
    • Glutamic Acid Decarboxylase 2 antibody
    • Glutamic Acid Decarboxylase 65 antibody
    • MGC161605 antibody
    • MGC161607 antibody
    see all

Anti-GAD65 antibody images

  • ab112007, at 2.5µg/ml, staining GAD65 in formalin-fixed, paraffin-embedded Human brain, cerebellum tissue by Immunohistochemistry.
  • ab112007, at 2.5µg/ml, staining GAD65 in formalin-fixed, paraffin-embedded Human colon tissue by Immunohistochemistry.
  • Anti-GAD65 antibody (ab112007) at 0.1 µg/ml + Human brain lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 65 kDa

References for Anti-GAD65 antibody (ab112007)

ab112007 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Guinea pig Tissue sections (Brain Tissue ( Hippo section))
Antigen retrieval step
Other
Permeabilization
No
Specification
Brain Tissue ( Hippo section)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.4% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 10 2016

The antigen retrieval step is usually performed after the fixation step to help relase the antigen. Fixing reduces the antigenicity of some cell components as the crosslinking caused by fixatives can obstruct antibody binding. Sometimes permeabilizatio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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