Overview

  • Product name
    Anti-GAD65 + GAD67 antibody
    See all GAD65 + GAD67 primary antibodies
  • Description
    Rabbit polyclonal to GAD65 + GAD67
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, ELISAmore details
    Unsuitable for: IHC-FoFr
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cat, Human, Zebrafish, Macaque monkey
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide:

    DFLIEEIERLGQDL

    , corresponding to C terminal amino acids 572-585 of Human GAD65+67.

  • Positive control
    • Brain.
  • General notes
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    An immunoglobulin-rich fraction was prepared from whole serum by ammonium sulphate precipitation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 20650017
IHC-Fr 1/200 - 1/5000.
WB 1/1000 - 1/10000. Predicted molecular weight: 65 , 67 kDa.
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-FoFr.
  • Target

    Images

    • ab11070 staining GABA transporter 2 in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab11070 at 1/200 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. GAD65/67 expression can be observed in amacrine cells (in green).

      See Abreview

    References

    This product has been referenced in:
    • Zhou X  et al. Alpha7 nicotinic acetylcholine receptor agonist promotes retinal ganglion cell function via modulating GABAergic presynaptic activity in a chronic glaucomatous model. Sci Rep 7:1734 (2017). WB, IHC-Fr ; Rat . Read more (PubMed: 28496108) »
    • Xia Z  et al. Zebrafish slc30a10 deficiency revealed a novel compensatory mechanism of Atp2c1 in maintaining manganese homeostasis. PLoS Genet 13:e1006892 (2017). WB ; Zebrafish . Read more (PubMed: 28692648) »

    See all 25 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - PBS Triton 3x5mn RT
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Fixative
    Paraformaldehyde
    Username

    Yacine Tensaouti

    Verified customer

    Submitted Aug 02 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina, Amacrine cells)
    Specification
    Retina, Amacrine cells
    Permeabilization
    Yes - Triton X
    Fixative
    Paraformaldehyde
    Username

    Dr. Ryan Macdonald

    Verified customer

    Submitted Sep 03 2013

    Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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    Thank you for contacting us.

    We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable....

    Read More

    Gracias por contactarnos de nuevo.

    Siento mucho los problemas ocasionados por estos productos que no han funcionado como se esperaba.

    Para poder dar el soporte oportuno de los mismos y entender por qué han dado ese resultado,...

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    Thank you for your reply. I hope the increased antibody will help to label the cell bodies. If you are able to get specific signal, then you may be able to reduce the background by shortening your antigen retrieval or increasing the blocking time. If n...

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    Thank you for contacting us. I am sorry that this antibody has been giving no signal in your IHC. Could you please clarify a few points of your protocol?

    1) What type of samples are you working with? What species and tissue type? How long wer...

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    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. I would like to confirm that you are eligible for our testing discount program if the antibody has not yet been...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Quail Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% triton X
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Ms. Charlotte Cornil

    Verified customer

    Submitted Sep 30 2011

    Thank you for participating in our testing discount program. When you submit your abreivew, please be sure to note the testing discount code in the "additional notes" section. The website does have specific questions for you to answer as well as the no...

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    1-10 of 12 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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