Overview

  • Product name
    Anti-GAD65 + GAD67 antibody
    See all GAD65 + GAD67 primary antibodies
  • Description
    Rabbit polyclonal to GAD65 + GAD67
  • Specificity
    ab55412 detects endogenous levels of total GAD65 and GAD67 protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from human GAD65 + GAD67.

  • Positive control
    • Mouse brain cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab55412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ELISA 1/5000.

Target

  • Relevance
    This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome.
  • Cellular localization
    Cytoplasm; cytosol. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
  • Database links
  • Alternative names
    • 65 kDa glutamic acid decarboxylase antibody
    • 67 kDa glutamic acid decarboxylase antibody
    • GAD 65 antibody
    • GAD 67 antibody
    • GAD1 antibody
    • GAD2 antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 2 antibody
    • Glutamate decarboxylase 2 pancreatic islets and brain 65kDa antibody
    • Glutamate decarboxylase 65 kDa isoform antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • SCP antibody
    see all

Images

  • All lanes : Anti-GAD65 + GAD67 antibody (ab55412) at 1/500 dilution

    Lane 1 : mouse brain lysate, without immunizing peptide
    Lane 2 : mouse brain lysate, with immunizing peptide


    Predicted band size : 65 kDa
    Observed band size : 65 kDa

References

This product has been referenced in:
  • Laramée ME  et al. Congenital Anophthalmia and Binocular Neonatal Enucleation Differently Affect the Proteome of Primary and Secondary Visual Cortices in Mice. PLoS One 11:e0159320 (2016). Read more (PubMed: 27410964) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab55412.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up