Overview

  • Product nameAnti-GAD67 antibody
    See all GAD67 primary antibodies
  • Description
    Rabbit polyclonal to GAD67
  • Specificityab52249 detects endogenous levels of total GAD67 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human GAD67.

  • Positive control
    • LOVO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab52249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
ELISA 1/10000.

Target

  • FunctionCatalyzes the production of GABA.
  • Tissue specificityIsoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • Involvement in diseaseDefects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • Sequence similaritiesBelongs to the group II decarboxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041055 antibody
    • SCP antibody
    see all

Anti-GAD67 antibody images

  • All lanes : Anti-GAD67 antibody (ab52249) at 1/300 dilution

    Lane 1 : LOVO cell extract; without peptide
    Lane 2 : LOVO cell extract; with peptide


    Predicted band size : 67 kDa
    Observed band size : 67 kDa

References for Anti-GAD67 antibody (ab52249)

This product has been referenced in:
  • Ku TH  et al. Effect of honokiol on activity of GAD(65) and GAD(67) in the cortex and hippocampus of mice. Phytomedicine 18:1126-9 (2011). WB ; Mouse . Read more (PubMed: 21561750) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2 · Temperature: 22°C
Antigen retrieval step None
Sample Mouse Tissue sections (dorsl raphe coronal brain slices)
Specification dorsl raphe coronal brain slices
Permeabilization Yes - Triton-X 100
Fixative Paraformaldehyde
Username

Jason Teem

Verified customer

Submitted Nov 14 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"