Anti-GAD67 antibody (ab75712)

Overview

  • Product name
  • Description
    Chicken polyclonal to GAD67
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region of GAD67 (EC 4.1.1.15) shared between the mouse (P48318), rat (NP_058703) and human (Q99259) gene products conjugated to KLH.

  • General notes

    It is important to note that, in our hands, we saw only a subpopulation of GABAergic neurons in various mouse brain structures, including cerebral cortex and cerebellar cortex, labeled with our anti-GAD-67 antibody. This was seen multiple times, and following steps to increase antibody penetration into the tissue. Although the basis of this mysterious subpopulation is unclear, we suspect that the epitope specifically recognized by this antibody on GAD-67 may be cryptic or absent in some inhibitory neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab75712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/2000.
IHC-P 1/1000 - 1/2000.

Target

  • Function
    Catalyzes the production of GABA.
  • Tissue specificity
    Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • Involvement in disease
    Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • Sequence similarities
    Belongs to the group II decarboxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041055 antibody
    • SCP antibody
    see all

Anti-GAD67 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of e18 mouse neurosphere cells in culture labelling GAD67 (green) with ab75712. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-MAP2, Blue: DAPI nuclear stain.

References for Anti-GAD67 antibody (ab75712)

ab75712 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry free floating
Sample
Zebra finch Tissue (Finch tissue sections (brain))
Specification
Finch tissue sections (brain)
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Submitted Sep 13 2016

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Mar 25 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
None
Sample
Rat Tissue sections (hippocampus)
Specification
hippocampus
Permeabilization
Yes - 0.3% trition
Fixative
Paraformaldehyde
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Submitted Oct 17 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
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Submitted Oct 17 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Chicken Tissue sections (hippocampus)
Specification
hippocampus
Permeabilization
Yes - 0.1% titon
Fixative
Paraformaldehyde
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Submitted Oct 17 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
None
Sample
Guinea pig Tissue sections (hippocampus)
Specification
hippocampus
Permeabilization
Yes - 0.2% triton
Fixative
Paraformaldehyde
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Submitted Oct 17 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Guinea pig Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
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Submitted Oct 15 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step
None
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
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Verified customer

Submitted Oct 15 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
None
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 15 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 15 2013

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