Anti-GAD67 antibody (ab75712)

Overview

  • Product nameAnti-GAD67 antibody
    See all GAD67 primary antibodies
  • Description
    Chicken polyclonal to GAD67
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region of GAD67 (EC 4.1.1.15) shared between the mouse (P48318), rat (NP_058703) and human (Q99259) gene products conjugated to KLH.

  • General notes

    It is important to note that, in our hands, we saw only a subpopulation of GABAergic neurons in various mouse brain structures, including cerebral cortex and cerebellar cortex, labeled with our anti-GAD-67 antibody. This was seen multiple times, and following steps to increase antibody penetration into the tissue. Although the basis of this mysterious subpopulation is unclear, we suspect that the epitope specifically recognized by this antibody on GAD-67 may be cryptic or absent in some inhibitory neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab75712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/2000.
IHC-P 1/1000 - 1/2000.

Target

  • FunctionCatalyzes the production of GABA.
  • Tissue specificityIsoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • Involvement in diseaseDefects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • Sequence similaritiesBelongs to the group II decarboxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041055 antibody
    • SCP antibody
    see all

Anti-GAD67 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of e18 mouse neurosphere cells in culture labelling GAD67 (green) with ab75712. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-MAP2, Blue: DAPI nuclear stain.

References for Anti-GAD67 antibody (ab75712)

ab75712 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry free floating
Sample Zebra finch Tissue (Finch tissue sections (brain))
Specification Finch tissue sections (brain)
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Submitted Sep 13 2016

Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 30 µg
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Mar 25 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step None
Sample Rat Tissue sections (hippocampus)
Specification hippocampus
Permeabilization Yes - 0.3% trition
Fixative Paraformaldehyde
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Submitted Oct 17 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step None
Sample Mouse Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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Submitted Oct 17 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Chicken Tissue sections (hippocampus)
Specification hippocampus
Permeabilization Yes - 0.1% titon
Fixative Paraformaldehyde
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Submitted Oct 17 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step None
Sample Guinea pig Tissue sections (hippocampus)
Specification hippocampus
Permeabilization Yes - 0.2% triton
Fixative Paraformaldehyde
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Submitted Oct 17 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Guinea pig Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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Submitted Oct 15 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step None
Sample Chicken Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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Submitted Oct 15 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step None
Sample Rat Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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Submitted Oct 15 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step None
Sample Mouse Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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Submitted Oct 15 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"