Overview

  • Product nameAnti-GAD67 antibody
    See all GAD67 primary antibodies
  • Description
    Goat polyclonal to GAD67
  • Specificityab80589 is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2 (isoform GAD65).
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Cat, Dog, Pig, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide:

    C-PDSPQRREKLHK

    , corresponding to internal sequence amino acids 526 - 537 of Human GAD67 (NP_000808.2)

  • Positive control
    • Mouse brain lysate, Rat brain lysate and Human brain (cerebellum) lysate. IHC-P: Human hippocampus.

Properties

Applications

Our Abpromise guarantee covers the use of ab80589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 67 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-GAD67 antibody images

  • IHC image of GAD67 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80589, 1µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-GAD67 antibody (ab80589) at 1 µg/ml + Mouse Brain lysate (in RIPA buffer) at 35 µg

    Predicted band size : 67 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Anti-GAD67 antibody (ab80589) at 0.5 µg/ml + Human brain (cerebral cortex) lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 67 kDa
    Observed band size : 67 kDa

References for Anti-GAD67 antibody (ab80589)

This product has been referenced in:
  • Taylor SR  et al. GABAergic and glutamatergic efferents of the mouse ventral tegmental area. J Comp Neurol 522:3308-34 (2014). IHC-FrFl ; Mouse . Read more (PubMed: 24715505) »

See 1 Publication for this product

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DISCOUNT CODE: 100%ABR-T76WH
Expiration date: 08-11-2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"