Anti-GAD67/GAD1 antibody (ab97739)
Key features and details
- Rabbit polyclonal to GAD67/GAD1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-GAD67/GAD1 antibody
See all GAD67/GAD1 primary antibodies -
Description
Rabbit polyclonal to GAD67/GAD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Cat, Dog, Pig, Zebrafish -
Immunogen
Recombinant fragment within Human GAD1/GAD67 aa 1-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q99259-1 -
Positive control
- H1299, HeLa, HepG2, Molt-4, Raji and NIH-3T3 cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 67 kDa.
|
|
ICC/IF | (1) |
1/100 - 1/200.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 67 kDa. |
ICC/IF
1/100 - 1/200. |
Target
-
Function
Catalyzes the production of GABA. -
Tissue specificity
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. -
Involvement in disease
Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture. -
Sequence similarities
Belongs to the group II decarboxylase family. - Information by UniProt
-
Database links
- Entrez Gene: 493699 Cat
- Entrez Gene: 517552 Cow
- Entrez Gene: 478794 Dog
- Entrez Gene: 2571 Human
- Entrez Gene: 14415 Mouse
- Entrez Gene: 396928 Pig
- Entrez Gene: 24379 Rat
- Entrez Gene: 378441 Zebrafish
see all -
Alternative names
- 67 kDa glutamic acid decarboxylase antibody
- CPSQ1 antibody
- DCE1 antibody
see all
Images
-
Anti-GAD67/GAD1 antibody (ab97739) at 1/500 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 67 kDa
7.5% SDS PAGE -
Anti-GAD67/GAD1 antibody (ab97739) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 67 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GAD1/GAD67 antibody (ab97739) at 1/200 dilution. Lower image was also merged with DNA probe.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (10)
ab97739 has been referenced in 10 publications.
- Ji NN et al. Microglia-derived TNF-α inhibiting GABAergic neurons in the anterior lateral bed nucleus of the stria terminalis precipitates visceral hypersensitivity induced by colorectal distension in rats. Neurobiol Stress 18:100449 (2022). PubMed: 35521065
- Krishnan V et al. Peripheral Nerve Injury Induces Changes in the Activity of Inhibitory Interneurons as Visualized in Transgenic GAD1-GCaMP6s Rats. Biosensors (Basel) 12:N/A (2022). PubMed: 35735531
- Chen X et al. Entorhinal cortex-based metabolic profiling of chronic restraint stress mice model of depression. Aging (Albany NY) 12:3042-3052 (2020). PubMed: 32074509
- Zhao X et al. Anodal and cathodal tDCS modulate neural activity and selectively affect GABA and glutamate syntheses in the visual cortex of cats. J Physiol 598:3727-3745 (2020). PubMed: 32506434
- Nobili A et al. Ambra1 Shapes Hippocampal Inhibition/Excitation Balance: Role in Neurodevelopmental Disorders. Mol Neurobiol 55:7921-7940 (2018). PubMed: 29488136
- Ding Y et al. Changes in GABAergic markers accompany degradation of neuronal function in the primary visual cortex of senescent rats. Sci Rep 7:14897 (2017). WB . PubMed: 29097694
- Casalia ML et al. Persistent seizure control in epileptic mice transplanted with gamma-aminobutyric acid progenitors. Ann Neurol 82:530-542 (2017). PubMed: 28833459
- Moraga A et al. Aging increases microglial proliferation, delays cell migration, and decreases cortical neurogenesis after focal cerebral ischemia. J Neuroinflammation 12:87 (2015). IF ; Mouse . PubMed: 25958332
- Stansfield KH et al. Early-life lead exposure recapitulates the selective loss of parvalbumin-positive GABAergic interneurons and subcortical dopamine system hyperactivity present in schizophrenia. Transl Psychiatry 5:e522 (2015). WB . PubMed: 25756805
- Bajo VM et al. The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex. Eur J Neurosci 40:2922-40 (2014). IHC ; Ferret . PubMed: 24945075