Overview

  • Product nameAnti-GAD67 antibody
    See all GAD67 primary antibodies
  • Description
    Rabbit polyclonal to GAD67
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 5 and 176 of GAD67.

  • Positive control
    • H1299, HeLa, HepG2, Molt-4, Raji and NIH-3T3 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 67 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionCatalyzes the production of GABA.
  • Tissue specificityIsoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
  • Involvement in diseaseDefects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.
  • Sequence similaritiesBelongs to the group II decarboxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 67 kDa glutamic acid decarboxylase antibody
    • CPSQ1 antibody
    • DCE1 antibody
    • DCE1_HUMAN antibody
    • EC 4.1.1.15 antibody
    • FLJ45882 antibody
    • GAD 67 antibody
    • GAD antibody
    • GAD-67 antibody
    • GAD1 antibody
    • Glutamate decarboxylase 1 (brain, 67kDa) antibody
    • Glutamate decarboxylase 1 antibody
    • Glutamate decarboxylase 1 brain 67kD antibody
    • Glutamate decarboxylase 1 brain 67kDa antibody
    • Glutamate decarboxylase 67 kDa isoform antibody
    • Glutamate decarboxylase, brain, 67-KD antibody
    • OTTHUMP00000041055 antibody
    • SCP antibody
    see all

Anti-GAD67 antibody images

  • Anti-GAD67 antibody (ab97739) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 67 kDa
  • Anti-GAD67 antibody (ab97739) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size : 67 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GAD67 antibody (ab97739) at 1/200 dilution. Lower image was also merged with DNA probe.

References for Anti-GAD67 antibody (ab97739)

This product has been referenced in:
  • Moraga A  et al. Aging increases microglial proliferation, delays cell migration, and decreases cortical neurogenesis after focal cerebral ischemia. J Neuroinflammation 12:87 (2015). IF ; Mouse . Read more (PubMed: 25958332) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Brain)
Permeabilization Yes - Triton-X
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Dr. S Jarrin

Verified customer

Submitted Jul 11 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain Tissue 40um slices)
Antigen retrieval step None
Permeabilization Yes - Triton X in PBS
Specification Brain Tissue 40um slices
Blocking step Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 24 2015

Thank you very much for your reply and for sending the additional information.

We have only tested the antibody in Western blot and in IF staining on fixed cells, so we aren't sure at this time whether it is suitable for use on perfused-fixed...

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Thank you very much for contacting us and letting us know about the trouble with this antibody.

I am sorry to hear that the results have not been satisfactory when using ab97739. We didupdate the immunogen region that was previously listed on...

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DISCOUNT CODE: 100%ABR-T76WH
Expiration date: 08-11-2012

I am very pleased to hear you would like to use ab97739 inIHC-FoFr and send us an image of your results.

This code will give you 1 free [PRIMARY ANTIBODY] before the expirat...

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Thank you for your inquiry.

Unfortunately, the blocking peptide forab97739 is not available for purchase.

Please let me know if there is anything else I can help you with.

Thank you for contacting us.

Your credit note ID for the refund is xxx.

I am sorry that this antibody did not have the correct immunogen informationon the datasheet, which we have corrected in the meantime. If payment has already ...

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Thank you for contacting us.

I have had the opportunity to inquire about the availability of the GAD76 blocking peptide as you had requested. Unfortunately I have been told that this is not presently available. While this control is not avail...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"