Overview

  • Product nameAnti-GADD34 antibody
    See all GADD34 primary antibodies
  • Description
    Rabbit polyclonal to GADD34
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 116-165 (GMYGEREATS VPRGQGSQFA DGQRAPLSPS LLIRTLQGSD KNPGEEKAEE) of the Human GADD34 protein (NP_055145).

  • Positive control
    • Human fetal heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab125464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRecruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.
  • Sequence similaritiesBelongs to the PPP1R15 family.
  • Post-translational
    modifications
    Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.
  • Cellular localizationEndoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis associated protein antibody
    • GADD 34 antibody
    • Growth arrest and DNA damage inducible 34 antibody
    • Growth arrest and DNA damage-inducible protein GADD34 antibody
    • Myeloid differentiation primary response protein MyD116 homolog antibody
    • Ppp1r15a antibody
    • PR15A_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 15A antibody
    • Protein phosphatase 1 regulatory subunit 15A antibody
    see all

Anti-GADD34 antibody images

  • Anti-GADD34 antibody (ab125464) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Predicted band size : 73 kDa

References for Anti-GADD34 antibody (ab125464)

ab125464 has not yet been referenced specifically in any publications.

Product Wall

Thank you for providing that information.
Based on all of the data that you have sent, I do not believe that there are any protocol variations that I can suggest that will help to resolve this issue. Therefore i would be happy to replace or refund...

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Thank you for contacting Abcam.
The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"