Overview

  • Product name
  • Description
    Rabbit polyclonal to GADD34
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 95-272 of Human GADD34 (O75807).

  • Positive control
    • 293T, A43, Jurkat and Raji whole cell lysates. IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab126075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 73 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.
  • Sequence similarities
    Belongs to the PPP1R15 family.
  • Post-translational
    modifications
    Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.
  • Cellular localization
    Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis associated protein antibody
    • GADD 34 antibody
    • Growth arrest and DNA damage inducible 34 antibody
    • Growth arrest and DNA damage-inducible protein GADD34 antibody
    • Myeloid differentiation primary response protein MyD116 homolog antibody
    • Ppp1r15a antibody
    • PR15A_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 15A antibody
    • Protein phosphatase 1 regulatory subunit 15A antibody
    see all

Images

  • ICC/IF image of ab126075 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126075, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-GADD34 antibody (ab126075) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 73 kDa
    7.5% SDS PAGE

References

ab126075 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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