Overview

  • Product nameAnti-GADD34 antibody
    See all GADD34 primary antibodies
  • Description
    Goat polyclonal to GADD34
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: AAALDLSGRRG., corresponding to C terminal amino acids 664-674 of Human GADD34.

  • Positive control
    • U937 lysate
  • General notesGenBank Accession Number – NP_055145 LocusLink ID - 23645

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).Can be blocked with Human GADD34 peptide (ab23166).

Target

  • FunctionRecruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'.
  • Sequence similaritiesBelongs to the PPP1R15 family.
  • Post-translational
    modifications
    Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity.
  • Cellular localizationEndoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis associated protein antibody
    • GADD 34 antibody
    • Growth arrest and DNA damage inducible 34 antibody
    • Growth arrest and DNA damage-inducible protein GADD34 antibody
    • Myeloid differentiation primary response protein MyD116 homolog antibody
    • Ppp1r15a antibody
    • PR15A_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 15A antibody
    • Protein phosphatase 1 regulatory subunit 15A antibody
    see all

Anti-GADD34 antibody images

  • Anti-GADD34 antibody (ab9869) at 0.1 µg/ml + HEPG2 cell lysate at 35 µg

    Predicted band size : 75 kDa
  • IHC image of ab9869 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9869, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-GADD34 antibody (ab9869)

This product has been referenced in:
  • Galindo I  et al. The ATF6 branch of unfolded protein response and apoptosis are activated to promote African swine fever virus infection. Cell Death Dis 3:e341 (2012). WB . Read more (PubMed: 22764100) »
  • Panaretakis T  et al. Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death. EMBO J 28:578-90 (2009). WB ; Mouse . Read more (PubMed: 19165151) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HL60)
Loading amount 10 µg
Specification HL60
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Jun 19 2007

Many thanks for you enquiry regarding GADD34 antibody. We aren't very sure whether we understood the question correctly. If its a problem with sensitivity, on the cell lysate we tested (U937)mentioned in the datsheet, we repeatedly achieved the signal....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"