Anti-GADD45A antibody - Aminoterminal end (ab76664)

Overview

  • Product name
    Anti-GADD45A antibody - Aminoterminal end
    See all GADD45A primary antibodies
  • Description
    Rabbit polyclonal to GADD45A - Aminoterminal end
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the N terminal region of human GADD45A.

  • Positive control
    • Y79 cell line lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 23706118
WB 1/50 - 1/100. Predicted molecular weight: 18 kDa.
ELISA 1/1000.

Target

  • Function
    Binds to proliferating cell nuclear antigen. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.
  • Sequence similarities
    Belongs to the GADD45 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDIT 1 antibody
    • DDIT-1 antibody
    • DDIT1 antibody
    • DNA damage inducible transcript 1 antibody
    • DNA damage-inducible transcript 1 protein antibody
    • GA45A_HUMAN antibody
    • GADD45 antibody
    • GADD45A antibody
    • Growth arrest and DNA damage inducible 45 alpha antibody
    • Growth arrest and DNA damage inducible alpha antibody
    • Growth arrest and DNA damage-inducible protein GADD45 alpha antibody
    see all

Anti-GADD45A antibody - Aminoterminal end images

  • Anti-GADD45A antibody - Aminoterminal end (ab76664) at 1/1000 dilution + Y79 whole cell lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 18 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

References for Anti-GADD45A antibody - Aminoterminal end (ab76664)

This product has been referenced in:
  • Spassov S  et al. Genetic targets of hydrogen sulfide in ventilator-induced lung injury--a microarray study. PLoS One 9:e102401 (2014). WB ; Mouse . Read more (PubMed: 25025333) »
  • Tront JS  et al. Gadd45a levels in human breast cancer are hormone receptor dependent. J Transl Med 11:131 (2013). IHC-P ; Human . Read more (PubMed: 23706118) »

See all 3 Publications for this product

Product Wall

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (human cell line CHRF)
Total protein in input
1000 µg
Immuno-precipitation step
Protein A/G
Specification
human cell line CHRF
Username

Abcam user community

Verified customer

Submitted Jun 26 2015

Thank you for contacting us.

We do not have a Gadd45a antibody that has been tested for IP. I was going to recommend ab76664 as the best option, assuming the IHC review that a customer sent was positive. (Positive IHC results suggest an anti...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast Cancer)
Specification
Breast Cancer
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

Submitted Dec 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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