Overview

  • Product nameAnti-GADD45G antibody
    See all GADD45G primary antibodies
  • Description
    Mouse monoclonal to GADD45G
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-160 of Human GADD45G

Properties

Applications

Our Abpromise guarantee covers the use of ab56802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK.
  • Sequence similaritiesBelongs to the GADD45 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • CR6 antibody
    • Cytokine responsive protein CR6 antibody
    • Cytokine-responsive protein CR6 antibody
    • DDIT-2 antibody
    • DDIT2 antibody
    • DNA damage-inducible transcript 2 protein antibody
    • GA45G_HUMAN antibody
    • Gadd45g antibody
    • Growth arrest and DNA damage inducible gamma antibody
    • Growth arrest and DNA damage-inducible protein GADD45 gamma antibody
    • GRP17 antibody
    see all

Anti-GADD45G antibody images

  • Western blot against tagged recombinant protein immunogen using ab56802 GADD45G antibody at 1ug/ml. Predicted band size of immunogen is 44 kDa
  • Overlay histogram showing JEG3 cells stained with ab56802 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56802, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-GADD45G antibody (ab56802)

ab56802 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. The Western blot data we presented is with a recombinant protein with GST tag. (MW of the GST tag alone is 26 KDa.) So, the antibody picked up a band at 43.6KDa. I hope this information will be helpful. Should you have a...

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