Overview

  • Product nameAnti-GAGE1 antibody
  • Description
    Mouse polyclonal to GAGE1
  • Specificityab67412 is reactive against mammalian transfected lysate.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-139 of Human GAGE1

  • Positive control
    • GAGE1 Transfected 293T lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 15 kDa).

Target

  • RelevanceThe GAGE1 gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The GAGE1 cDNA contains a 143-bp insertion, located in the coding sequence near the termination codon, that is absent from the other cDNAs. Autologous cytolytic T lymphocytes recognise the antigenic peptide YRPRPRRY, which is also encoded by several other family members. Nothing is presently known about the function of this protein. An alternatively spliced transcript variant has been found for this gene.
  • Database links
  • Alternative names
    • Cancer/testis antigen 4.1 antibody
    • CT4 1 antibody
    • G antigen 1 antibody
    • GAGE 1 antibody
    • MZ2 F antigen antibody
    see all

Anti-GAGE1 antibody images

  • All lanes : Anti-GAGE1 antibody (ab67412) at 1/500 dilution

    Lane 1 : GAGE1 transfected 293T lysate
    Lane 2 : Non transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 15 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)

References for Anti-GAGE1 antibody (ab67412)

This product has been referenced in:
  • Nylund C  et al. Melanoma-Associated Cancer-Testis Antigen 16 (CT16) Regulates the Expression of Apoptotic and Antiapoptotic Genes and Promotes Cell Survival. PLoS One 7:e45382 (2012). WB . Read more (PubMed: 23028975) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"