Overview

  • Product nameAnti-GAJ antibody
    See all GAJ primary antibodies
  • Description
    Mouse polyclonal to GAJ
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length GAJ protein (Human)

  • Positive control
    • Human H1 skeletal muscle tissue lysate and 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe product of the GAJ gene associates with HOP2 to form a stable heterodimeric complex that binds DNA and stimulates the recombinase activity of RAD51 and DMC1 (Chi et al., 2007 [PubMed 17639080]). Both the MND1 and HOP2 genes are indispensable for meiotic recombination.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • 2610034E18Rik antibody
      • AI591601 antibody
      • GAJ protein antibody
      • Homolog of yeast MND1 antibody
      • Meiotic nuclear division 1, S. cerevisiae. homolog of antibody
      • Meiotic nuclear division protein 1 homolog antibody
      • Meiotic nuclear divisions 1 homolog (S. cerevisiae) antibody
      • Meiotic nuclear divisions 1 homolog antibody
      • MGC134110 antibody
      • MND1 antibody
      • RGD1309007 antibody
      see all

    Anti-GAJ antibody images

    • Anti-GAJ antibody (ab67124) at 1/500 dilution + human H1 skeletal muscle tissue lysate at 25 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 24 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-GAJ antibody (ab67124) at 1/500 dilution

      Lane 1 : GAJ transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 24 kDa
      Observed band size : 24 kDa

    References for Anti-GAJ antibody (ab67124)

    ab67124 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab67124.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"