Anti-GAK antibody [1C2] (ab115179)

Overview

  • Product nameAnti-GAK antibody [1C2]
    See all GAK primary antibodies
  • Description
    Mouse monoclonal [1C2] to GAK
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant Rat GAK

  • Positive control
    • Jurkat, Raji, MCF7, U937, HL60, HepG, WR19L12a and NIH3T3 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab115179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 144 kDa. (Colorimetric).

Target

  • FunctionAssociates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
  • Tissue specificityUbiquitous. Highest in testis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 C2 tensin-type domain.
    Contains 1 J domain.
    Contains 1 phosphatase tensin-type domain.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm > perinuclear region. Golgi apparatus > trans-Golgi network. Cell junction > focal adhesion. Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focals adhesions.
  • Information by UniProt
  • Database links
  • Alternative names
    • Auxilin 2 antibody
    • Cyclin G associated kinase antibody
    • Cyclin-G-associated kinase antibody
    • DNAJ26 antibody
    • DNAJC26 antibody
    • EC 2.7.11.1 antibody
    • FLJ16629 antibody
    • FLJ40395 antibody
    • GAK antibody
    • GAK protein antibody
    • GAK_HUMAN antibody
    • MGC99654 antibody
    see all

Anti-GAK antibody [1C2] images

  • All lanes : Anti-GAK antibody [1C2] (ab115179) at 1 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : U937 cell lysate
    Lane 5 : HL60 cell lysate
    Lane 6 : HepG2 cell lysate
    Lane 7 : WR19L12a cell lysate
    Lane 8 : NIH3T3 cell lysate


    Predicted band size : 144 kDa

References for Anti-GAK antibody [1C2] (ab115179)

ab115179 has not yet been referenced specifically in any publications.

Product Wall

The immunogen for ab115179 is recombinant rat full-length GAK GST-fusion protein corresponding to Uniprot ID, http://www.uniprot.org/uniprot/P97874. We do not know of any epitope mapping studies that have determined specifically what portion of the GAK...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"