Overview

  • Product name
    Anti-GAK protein antibody
  • Description
    Rabbit polyclonal to GAK protein
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human GAK aa 15-45 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q6P490

  • Positive control
    • Mouse heart tissue lysate. Human hepatocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 62 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Relevance
    GAK, a member of the Ser/Thr protein kinase family, associates with cyclin G and CDK5. It appears to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. GAK localizes to the perinuclear area and to the trans-Golgi network. It is also observed on the plasma membrane, probably at focals adhesions. Expression is ubiquitous, wiht highest levels in testis. The protein contains 1 J domain and 1 tensin domain. (Information taken form the supplier datasheet for ab86397)
  • Database links

Images

  • Anti-GAK protein antibody (ab86397) at 1/100 dilution + mouse heart tissue lysate at 12.5 µg

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • ab86397 at a 1/1000 dilution staining GAK in hepatocarcinoma tissue. DAB staining.

References

ab86397 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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