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Gal 4 activation domain mini protein (amino acids 768-881) tagged to Maltose binding protein.
The GAL4 Activation Domain monoclonal antibody binds specifically to the AD of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of fusion proteins containing GAL4 AD as the fusion partner in two hybrid studies. The activation domain is typically used in a two-hybrid experiment as the fusion partner for a library of cDNA clones to screen against a protein of interest fused to a DNA-binding domain. Gal4is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
Product was previously marketed under the MitoSciences sub-brand.
Our Abpromise guarantee covers the use of ab135398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 99 kDa.|
|Other||Use at an assay dependent concentration. Yeast two hybrid studies|
ab135398 has not yet been referenced specifically in any publications.