Anti-GAL4 antibody [14-7E10G10] (ab135398)

Overview

  • Product nameAnti-GAL4 antibody [14-7E10G10]
    See all GAL4 primary antibodies
  • Description
    Mouse monoclonal [14-7E10G10] to GAL4
  • Tested applicationsWB, Othermore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Gal 4 activation domain mini protein (amino acids 768-881) tagged to Maltose binding protein.

  • General notes


    The GAL4 Activation Domain monoclonal antibody binds specifically to the AD of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of fusion proteins containing GAL4 AD as the fusion partner in two hybrid studies. The activation domain is typically used in a two-hybrid experiment as the fusion partner for a library of cDNA clones to screen against a protein of interest fused to a DNA-binding domain. Gal4is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.02% Sodium azide
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Primary antibody notes The GAL4 Activation Domain monoclonal antibody binds specifically to the AD of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of fusion proteins containing GAL4 AD as the fusion partner in two hybrid studies. The activation domain is typically used in a two-hybrid experiment as the fusion partner for a library of cDNA clones to screen against a protein of interest fused to a DNA-binding domain. Gal4is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
  • Clonality Monoclonal
  • Clone number14-7E10G10
  • IsotypeIgG2b
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab135398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 99 kDa.
Other Use at an assay dependent concentration. Yeast two hybrid studies

Target

  • RelevanceFunction: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
      • Gal4p antibody
      • GAL81 antibody
      • Regulatory protein GAL4 antibody

    Anti-GAL4 antibody [14-7E10G10] images

    • All lanes : Anti-GAL4 antibody [14-7E10G10] (ab135398) at 0.5 µg/ml

      Lane 1 : Whole cell yeast lysate control from untransformed SEY6210 pep4-3 strain (a kind gift from Prof. Tom Stevens, University of Oregon).
      Lane 2 : Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJK22-pGBDU plasmid containing GAL4 DNA Binding domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).
      Lane 3 : Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJk23-pGAD plasmid containing GAL4 Active Domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).

      Secondary
      Lane 1 : Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) - 1:10000
      Lane 2 : Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP)- 1:10000
      Lane 3 : Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) - 1:10000


      Predicted band size : 99 kDa
      Additional bands at : 56 kDa (possible Fusion protein).

    References for Anti-GAL4 antibody [14-7E10G10] (ab135398)

    ab135398 has not yet been referenced specifically in any publications.

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