Overview

  • Product nameAnti-GAL4 antibody [3G152]
    See all GAL4 primary antibodies
  • Description
    Mouse monoclonal [3G152] to GAL4
  • SpecificityThis antibody can be used to detect both N-terminal and C-terminal tagged Gal4(1–147) and Gal4(1–92) fusion proteins.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WB, ICCmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-147 of S. cerevisiae GAL4.

  • EpitopeThe immunoreactive epitope maps within amino acids 1–92 of GAL4.
  • Positive control
    • N-terminal and C-terminal tagged GAL4 (1-147) and GAL4 (1-92) fusion proteins

Properties

Applications

Our Abpromise guarantee covers the use of ab14477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20610754
ELISA Use a concentration of 0.1 - 1 µg/ml.
WB Use a concentration of 3 µg/ml. Predicted molecular weight: 99 kDa.
ICC Use at an assay dependent concentration.

Target

  • RelevanceFunction: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
      • Gal4p antibody
      • GAL81 antibody
      • Regulatory protein GAL4 antibody

    References for Anti-GAL4 antibody [3G152] (ab14477)

    This product has been referenced in:
    • Chen Y  et al. Intramembranous valine linked to schizophrenia is required for neuregulin 1 regulation of the morphological development of cortical neurons. J Neurosci 30:9199-208 (2010). ICC/IF . Read more (PubMed: 20610754) »

    See 1 Publication for this product

    Product Wall

    Thisantibody has only been tested with GAL4-fusion proteins inWestern Blot.

    I hope this is helpful. Please contact me again if you have any further questions.

    Application Immunocytochemistry
    Sample Human Recombinant protein (Transfected cells)
    Specification Transfected cells
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted Aug 17 2005

    Thank you for contacting us for technical support and taking the time to fill in our protocol questionnaire, I'm very sorry to hear you are experiencing problems with ab14477 in your samples. I have looked at your order details and can confirm ther...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"