Anti-GAL4 antibody [5C8] (ab16157)

Overview

  • Product name
    Anti-GAL4 antibody [5C8]
    See all GAL4 primary antibodies
  • Description
    Mouse monoclonal [5C8] to GAL4
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Full length protein corresponding to Saccharomyces cerevisiae GAL4.

  • Epitope
    ab16157 recognizes GAL4 derivatives containing the C-terminal activation domain.

Applications

Our Abpromise guarantee covers the use of ab16157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • Relevance
    Function: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • Gal4p antibody
      • GAL81 antibody
      • Regulatory protein GAL4 antibody

    References

    ab16157 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your enquiyr. This particular antibody reacts with the DNA activation region of the GAL-4 protein.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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