Overview

  • Product nameAnti-GAL4 antibody
    See all GAL4 primary antibodies
  • Description
    Rabbit polyclonal to GAL4
  • Specificityab50149 recognizes GAL4 DNA binding domain fusion proteins
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to Saccharomyces cerevisiae GAL4 aa 39-52 conjugated to keyhole limpet haemocyanin.
    Sequence:

    RYSPKTKRSPLTRA

  • Positive control
    • GAL4 DNA-BD fusion protein in Saccharomyces cervisiae extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab50149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 99 kDa.

Target

  • RelevanceFunction: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
      • Gal4p antibody
      • GAL81 antibody
      • Regulatory protein GAL4 antibody

    References for Anti-GAL4 antibody (ab50149)

    ab50149 has not yet been referenced specifically in any publications.

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