Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling GAL4 with ab89559. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 99 kDa
References for Anti-GAL4 antibody [MM0300-1D12] (ab89559)
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