Overview

  • Product name
    Galanin (1-29) (rat, mouse)
  • Description
    Non-selective galanin receptor agonist
  • Biological description
    Non-selective galanin receptor agonist (Ki values are 0.98, 1.48 and 1.47 nM at GAL1, GAL2 and GAL3, respectively). Anticonvulsant and neuroprotective effects in vivo.
  • Purity
    > 95%

Properties

Applications

Our Abpromise guarantee covers the use of ab141153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab81283 staining AKT1 (phospho S473) in PC12 cells treated with galanin (1-29) (rat, mouse) (ab141153), by ICC/IF. Increase of AKT1 (phospho S473) expression correlates with increased concentration of galanin (1-29) (rat, mouse), as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab141153 (galanin (1-29) (rat, mouse)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81283 (1/100) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Mazarati A  et al. Regulation of kindling epileptogenesis by hippocampal galanin type 1 and type 2 receptors: The effects of subtype-selective agonists and the role of G-protein-mediated signaling. J Pharmacol Exp Ther 318:700-8 (2006). Read more (PubMed: 16699066) »
  • Mazarati A & Lu X Regulation of limbic status epilepticus by hippocampal galanin type 1 and type 2 receptors. Neuropeptides 39:277-80 (2005). Read more (PubMed: 15944022) »
  • Branchek TA  et al. Galanin receptor subtypes. Trends Pharmacol Sci 21:109-17 (2000). Read more (PubMed: 10689365) »
  • Wang S  et al. Cloning and expressional characterization of a novel galanin receptor. Identification of different pharmacophores within galanin for the three galanin receptor subtypes. J Biol Chem 272:31949-52 (1997). Read more (PubMed: 9405385) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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