Overview

  • Product nameAnti-Galanin antibody
    See all Galanin primary antibodies
  • Description
    Mouse monoclonal to Galanin
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-124 of Human Galanin

  • Positive control
    • ICC/IF: SKNSH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab58274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionContracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion.
  • Sequence similaritiesBelongs to the galanin family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GAL 1 antibody
    • GAL antibody
    • GAL GMAP antibody
    • GAL1 antibody
    • GALA_HUMAN antibody
    • Galanin message associated peptide antibody
    • Galanin message-associated peptide antibody
    • Galanin precursor antibody
    • Galanin prepropeptide antibody
    • Galanin related peptide antibody
    • Galanin/GMAP prepropeptide antibody
    • GALN antibody
    • GLNN antibody
    • GMAP antibody
    • MGC40167 antibody
    see all

Anti-Galanin antibody images

  • ICC/IF image of ab58274 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58274, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot against tagged recombinant protein immunogen using ab58274 Galanin antibody at 1ug/ml. Predicted band size of immunogen is 39 kDa

References for Anti-Galanin antibody (ab58274)

ab58274 has not yet been referenced specifically in any publications.

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Expiration date: October 6th, 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"