Overview

  • Product name
  • Description
    Rabbit polyclonal to Galanin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Pig, Chimpanzee, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Galanin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P22466

  • Positive control
    • This antibody gave a positive signal in Human Pancreas tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab87378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 13 kDa).

Target

Images

  • Anti-Galanin antibody (ab87378) at 1 µg/ml + Pancreas (Human) Tissue Lysate - adult normal tissue at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 13 kDa
    Observed band size: 16 kDa (why is the actual band size different from the predicted?)


    Exposure time: 16 minutes


    The predicted molecular weight of Galanin is 13 kDa (SwissProt), however we expect to observe a banding pattern at 16 kDa.

    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab87378 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab87378 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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