Overview

  • Product name
  • Description
    Goat polyclonal to Galanin
  • Tested applications
    Suitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide:

    HRSFSDKNGLTSK

    by a Cysteine residue linker, corresponding to internal sequence amino acids 51-63 of Galanin (NP_057057.2)

  • Positive control
    • Human Brain (Cerebellum) lysate, Mouse Brain Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 13 kDa.
IHC-FoFr Use a concentration of 0.2 - 0.5 µg/ml.

Target

Anti-Galanin antibody images

  • Anti-Galanin antibody (ab99452) at 1 µg/ml + Human Cerebellum Lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 13 kDa
  • Immunohistochemical analysis of PFA-perfused frozen Human hypothalamus tissue, staining Galanin with ab99452 at 0.3 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).

References for Anti-Galanin antibody (ab99452)

This product has been referenced in:
  • Ippagunta SK  et al. The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization. Nat Immunol 12:1010-6 (2011). WB . Read more (PubMed: 21892172) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Mesenchymal Stem Cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mesenchymal Stem Cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Yen You Nick Chen

Verified customer

Submitted Dec 21 2016

I am very pleased to hear you would like to accept our offer and test ab99452 in IHC. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the “Ad...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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