Overview

  • Product name
  • Description
    Goat polyclonal to Galanin
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide:

    HRSFSDKNGLTSK

    by a Cysteine residue linker, corresponding to internal sequence amino acids 51-63 of Galanin (NP_057057.2)

  • Positive control
    • Human Brain (Cerebellum) lysate, Mouse Brain Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 13 kDa.
IHC-FoFr Use a concentration of 0.2 - 0.5 µg/ml.

Target

Images

  • Anti-Galanin antibody (ab99452) at 1 µg/ml + Human Cerebellum Lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 13 kDa

  • Immunohistochemical analysis of PFA-perfused frozen Human hypothalamus tissue, staining Galanin with ab99452 at 0.3 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).

References

This product has been referenced in:
  • Ippagunta SK  et al. The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization. Nat Immunol 12:1010-6 (2011). WB . Read more (PubMed: 21892172) »

See 1 Publication for this product

Customer reviews and Q&As

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Mesenchymal Stem Cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mesenchymal Stem Cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Yen You Nick Chen

Verified customer

Submitted Dec 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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