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Our Abpromise guarantee covers the use of ab25138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ELISA||Use a concentration of 0.5 µg/ml. Using 100 µl/well of antibody solution, allows the detection of 0.2-0.4 ng/well of recombinant human Galectin 1.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). The detection limit for recombinant human Galectin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
ab25138 staining Galectin 1 in Human umbilical cord blood mescenchymal stem cells by Flow Cytometry. Cells were fixed with formaldehyde. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 30 minutes at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polycloanl (1/2000) was used as the secondary antibody.
Purple - isotype control, Green - Galectin 1.