• Product nameAnti-Galectin 1 antibody
    See all Galectin 1 primary antibodies
  • Description
    Rabbit polyclonal to Galectin 1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cyt, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).



Our Abpromise guarantee covers the use of ab25138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ELISA Use a concentration of 0.5 µg/ml. Using 100 µl/well of antibody solution, allows the detection of 0.2-0.4 ng/well of recombinant human Galectin 1.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). The detection limit for recombinant human Galectin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.


  • FunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
  • Tissue specificityExpressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
  • Sequence similaritiesContains 1 galectin domain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 14 kDa laminin-binding protein antibody
    • 14 kDa lectin antibody
    • Beta galactoside binding lectin antibody
    • Beta galactoside binding lectin L 14 I antibody
    • beta galactoside binding protein antibody
    • Beta-galactoside-binding lectin L-14-I antibody
    • DKFZp686E23103 antibody
    • Gal 1 antibody
    • Gal-1 antibody
    • GAL1 antibody
    • Galaptin antibody
    • Galbp antibody
    • Galectin antibody
    • Galectin-1 antibody
    • Galectin1 antibody
    • GBP antibody
    • HBL antibody
    • HLBP14 antibody
    • HPL antibody
    • L 14.5 antibody
    • L-14.5 antibody
    • L14 antibody
    • Lactose binding lectin 1 antibody
    • Lactose-binding lectin 1 antibody
    • Lect14 antibody
    • Lectin galactoside binding soluble 1 antibody
    • Lectin galactoside-binding soluble 1 antibody
    • LEG1_HUMAN antibody
    • LGALS 1 antibody
    • LGALS1 antibody
    • Lgals1 lectin galactose binding soluble 1 antibody
    • MAPK activating protein MP12 antibody
    • Putative MAPK activating protein MP12 antibody
    • Putative MAPK-activating protein PM12 antibody
    • S Lac lectin 1 antibody
    • S-Lac lectin 1 antibody
    see all

Anti-Galectin 1 antibody images

  • ab25138 staining Galectin 1 in Human umbilical cord blood mescenchymal stem cells by Flow Cytometry. Cells were fixed with formaldehyde. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 30 minutes at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polycloanl (1/2000) was used as the secondary antibody.

    Purple - isotype control, Green - Galectin 1.

    See Abreview

  • ICC/IF image of ab25138 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25138, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab25138 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25138, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Galectin 1 antibody (ab25138) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 32 kDa,45 kDa,73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-Galectin 1 antibody (ab25138)

This product has been referenced in:
  • van Esch EM  et al. Expression of coinhibitory receptors on T cells in the microenvironment of usual vulvar intraepithelial neoplasia is related to proinflammatory effector T cells and an increased recurrence-free survival. Int J Cancer 136:E95-106 (2015). Read more (PubMed: 25220367) »
  • Punt S  et al. Galectin-1, -3 and -9 Expression and Clinical Significance in Squamous Cervical Cancer. PLoS One 10:e0129119 (2015). IHC-P ; Human . Read more (PubMed: 26066796) »

See all 5 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization No
Sample Human Cell (umbilical cord blood mescenchymal stem cell)
Specification umbilical cord blood mescenchymal stem cell
Gating Strategy we did not perform the gating step
Preparation Cell harvesting/tissue preparation method: detachment using trypsin
Sample buffer: PBS

Abcam user community

Verified customer

Submitted Jan 16 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (293T, Hela)
Specification 293T, Hela
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jun 24 2013

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

Read More

Pas de problème, j'ai donc demandé à notre service comptabilité de mettre en place un avoir, référence CN est ****** (pour le ab94571). Voici les manières dont vous pouvez utiliser cette référence CN: (1) la faire valoir contre la facture originale...

Read More

Merci pour ces informations. Je suis désolé d'apprendre qu'aucun des 2 anticorps de remplacement n'a fonctionné en western blot sur échantillons humains. Pour des raisons de qualité, j'ai transmis vos données et commentaires au laboratoire afin q...

Read More

DISCOUNT CODE: xxxxxxxxxxx Expiration date: 14-01-2012 I am very pleased to hear you would like to accept our offer and test ab25138 in Flow Cytometry. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this...

Read More

Thank you very much for your interest in ab25138. To our knowledge, ab25138 has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy ab25138 now, test it in Flow and submit feedback to us in the ...

Read More