Anti-Galectin 1 antibody [EPR3205] (ab108389)

Overview

  • Product nameAnti-Galectin 1 antibody [EPR3205]
    See all Galectin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3205] to Galectin 1
  • Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-FoFrmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Galectin 1

  • Positive control
    • HL60 cell lysate, K562 cell lysate, NIH 3T3 cell lysate, mouse heart tissue lysate, mouse kidney cell lysate, mouse spleen cell lysate, rat heart tissue lysate, rat kidney tissue lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab108389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IP 1/10 - 1/100.
ICC/IF 1/100 - 1/250.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23682281
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.
    • Tissue specificityExpressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
    • Sequence similaritiesContains 1 galectin domain.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 14 kDa laminin-binding protein antibody
      • 14 kDa lectin antibody
      • Beta galactoside binding lectin antibody
      • Beta galactoside binding lectin L 14 I antibody
      • beta galactoside binding protein antibody
      • Beta-galactoside-binding lectin L-14-I antibody
      • DKFZp686E23103 antibody
      • Gal 1 antibody
      • Gal-1 antibody
      • GAL1 antibody
      • Galaptin antibody
      • Galbp antibody
      • Galectin antibody
      • Galectin-1 antibody
      • Galectin1 antibody
      • GBP antibody
      • HBL antibody
      • HLBP14 antibody
      • HPL antibody
      • L 14.5 antibody
      • L-14.5 antibody
      • L14 antibody
      • Lactose binding lectin 1 antibody
      • Lactose-binding lectin 1 antibody
      • Lect14 antibody
      • Lectin galactoside binding soluble 1 antibody
      • Lectin galactoside-binding soluble 1 antibody
      • LEG1_HUMAN antibody
      • LGALS 1 antibody
      • LGALS1 antibody
      • Lgals1 lectin galactose binding soluble 1 antibody
      • MAPK activating protein MP12 antibody
      • Putative MAPK activating protein MP12 antibody
      • Putative MAPK-activating protein PM12 antibody
      • S Lac lectin 1 antibody
      • S-Lac lectin 1 antibody
      see all

    Anti-Galectin 1 antibody [EPR3205] images

    • All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution

      Lane 1 : HL60 cell lysate
      Lane 2 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
    • All lanes : Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution

      Lane 1 : mouse heart tissue lysate
      Lane 2 : mouse kidney tissue lysate
      Lane 3 : mouse spleen tissue lysate
      Lane 4 : rat heart tissue lysate
      Lane 5 : rat kidney tissue lysate
      Lane 6 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa

    References for Anti-Galectin 1 antibody [EPR3205] (ab108389)

    This product has been referenced in:
    • Pereira PM  et al. Galactodendritic phthalocyanine targets carbohydrate-binding proteins enhancing photodynamic therapy. PLoS One 9:e95529 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24763311) »
    • Gurel Z  et al. Identification of O-GlcNAc modification targets in mouse retinal pericytes: implication of p53 in pathogenesis of diabetic retinopathy. PLoS One 9:e95561 (2014). WB ; Mouse . Read more (PubMed: 24788674) »

    See all 4 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (human)
    Gel Running Conditions Reduced Denaturing (8%)
    Loading amount 50 µg
    Treatment alpha synuclein 3hr
    Specification human
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 30 2015



    As this is an Epitomics product, I have forwarded your request to them. They will be in catact with you shortly.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"