The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10 µl to label 106 cells. The antibody is suitable for indirect intracytoplasmic staining by flow cytometry.
Galectin 10 is an intracellular antigen. Fixation with paraformaldehyde 4% and permeabilisation with saponin 0.1% are required before staining.
ICC/IF: Use at an assay dependent dilution. The antibody is suitable for immunofluorescence staining on cytospins and cryosections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May have both lysophospholipase and carbohydrate-binding activities.
Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils.
Contains 1 galectin domain.
Cytoplasmic granule. Localized in granules from where it may be secreted or transported to other locations in the cell.
Immunocytochemistry/ Immunofluorescence - Galectin 10 antibody [B-F42] (ab27417)Image courtesy of an anonymous Abreview.
ab27417 staining Galectin 10 in human Eosinophil Granulocyte cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100 and then blocked using 10% serum for 30 minutes at 37°C. Samples were then incubated with primary antibody at 1/40 for 1 hour at 37°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Cy2® used at a 1/200 dilution.