Anti-Galectin 10 antibody [B-F42] (ab27417)

Overview

  • Product nameAnti-Galectin 10 antibody [B-F42]
    See all Galectin 10 primary antibodies
  • Description
    Mouse monoclonal [B-F42] to Galectin 10
  • SpecificityThis antibody is specific for both natural and recombinant human Galectin 10.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Galectin 10 protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab27417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
ICC/IF
  • Application notesFlow Cyt: Use 10 µl to label 106 cells. The antibody is suitable for indirect intracytoplasmic staining by flow cytometry.
    Galectin 10 is an intracellular antigen. Fixation with paraformaldehyde 4% and permeabilisation with saponin 0.1% are required before staining.

    ICC/IF: Use at an assay dependent dilution. The antibody is suitable for immunofluorescence staining on cytospins and cryosections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay have both lysophospholipase and carbohydrate-binding activities.
    • Tissue specificityExpressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils.
    • Sequence similaritiesContains 1 galectin domain.
    • Cellular localizationCytoplasmic granule. Localized in granules from where it may be secreted or transported to other locations in the cell.
    • Information by UniProt
    • Database links
    • Alternative names
      • BSF3 antibody
      • Charcot Leyden crystal protein antibody
      • Charcot-Leyden crystal protein antibody
      • CLC antibody
      • EC 3.1.1.5 antibody
      • Eosinophil lysophospholipase antibody
      • Gal-10 antibody
      • Galectin-10 antibody
      • LGALS10 antibody
      • LGALS10A antibody
      • LPPL HUMAN antibody
      • LPPL_HUMAN antibody
      • Lysolecithin acylhydrolase antibody
      • MGC149659 antibody
      see all

    Anti-Galectin 10 antibody [B-F42] images

    • ab27417 staining Galectin 10 in human Eosinophil Granulocyte cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100 and then blocked using 10% serum for 30 minutes at 37°C. Samples were then incubated with primary antibody at 1/40 for 1 hour at 37°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Cy2® used at a 1/200 dilution.

    References for Anti-Galectin 10 antibody [B-F42] (ab27417)

    ab27417 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Eosinophil Granulocyte)
    Specification Eosinophil Granulocyte
    Fixative Paraformaldehyde
    Permeabilization Yes - 0,5% Triton X-100
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
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    Submitted Apr 23 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"